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Microbiology Society, International Journal of Systematic and Evolutionary Microbiology, 3(66), p. 1306-1310, 2016

DOI: 10.1099/ijsem.0.000880

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Mariniluteicoccus endophyticus sp. nov., an endophytic actinobacterium isolated from root of Ocimum basilicum

This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Two Gram-staining-positive, aerobic, non-motile, irregular cocci strains, designated YIM 2617T and YIM 2617-2, were isolated from the root of Ocimum basilicum. Phylogenetic analysis on the basis of 16S rRNA gene sequence comparison revealed that the two strains were closely related to Mariniluteicoccus flavus YIM M13146T (98.1% and 98.0% sequence similarities, respectively) and formed a robust clade with M. flavus in the neighbour-joining tree. Optimum growth of the strains was observed at 28-32°C, pH 7.0-8.0 and in the presence of 0-2% NaCl (w/v). The chemotaxonomic profiles of the strains comprised of anteiso-C15:0 as the major cellular fatty acid, MK-9(H4) as the predominant menaquinone, alanine, glycine, glutamic acid and LL-diaminopimelic acid as peptidoglycan components, and phosphatidylcholine and diphosphatidylglycerol as the major polar lipids. The G+C contents of the genomic DNAs were 66.4 and 66.6 mol %, respectively. DNA-DNA hybridizations of the two strains with M. flavus YIM M13146T gave relatedness values of 47.6 ± 2.0 % and 43.2 ± 1.5 %, respectively, while the relatedness value between them was 92.1 ± 2.2 %. On the basis of the data recorded from the present study, strains YIM 2617T and YIM 2617-2 represent a novel species of the genus Mariniluteicoccus, for which the name Mariniluteicoccus endophyticus sp. nov. is proposed. The type strain is YIM 2617T (=KCTC 29482T = DSM 28728T= JCM 30097T).