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A Universal plasmonic/microfluidic platform for spatial and temporal controlled intracellular delivery is described. The system can inject/transfect the desired amount of molecules with an efficacy close to 100%. Moreover, it is highly scalable from single cells to large ensembles without administering the molecules to an extracellular bath. The latter enables quantitative control over the amount of injected molecules. © 2015 The Authors. Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. © 2015 he Authors. Published by ILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.