The aim of this study is to investigate the ability of flavan-3-ols: catechin, epigallocatechin, epigallocatechin gallate, and epicatechin gallate to prevent aflatoxin B1 (AB1)-induced cytotoxicity in A2780 cell line. The treatment with AB1 (0.35 μM) gives an increased level of cytotoxicity versus control (30-50%). Flavan-3-ols are more efficient against AB1 cytotoxicity at 24 h after treatment than after 48 h. AB1-induced dichlorofluorescin diacetate fluorescence intensity was significantly reduced even after 1 h incubation with flavan-3-ols. Concerning the tumor necrosis factor-alpha (TNF-α) release, comparing with the control, AB1 induces a down-expression of this factor. All the flavan-3-ols induced a higher level of TNF-α. release in the cellular supernatant comparing with the AB1 group, but much lower than the control group. The present study demonstrates the potent protective effect of flavan-3-ols against AB1 cytotoxicity in A2780 epithelial cell line. This protective effect may be due to the inhibitory effect of flavan-3-ols on AB1-induced reactive oxygen species formation and oxidative damage.