Two dimensional (2-D) structured membranes have been well developed and widely studied to find potential applications in broad realms like optics, mechanics, fluidics, and electronics. In this work, the authors have successfully combined the top–down patterning techniques with the roll-up process to convert various structured flat membranes into three dimensional (3-D) microtubes with textured tube-walls. These 3-D textured microtubes may exhibit novel properties different from the original 2-D films and, thus, can be applied in wider research disciplines such as modern material sciences, biology, electrochemistry, etc. Depending on the parameters of the periodic templates including nanoscale porous anodic alumina and microscale imprinted templates in this work, the authors can curve these textured films into 3-D microtubes with structures on the tube-walls by the rolled-up nanotechnique. The specially designed microtubes here have the potential of interesting optical, electrical, and mechanical characteristics as well as possible applications in micro/nanoelectronics, optics, fludics, and bioengineering.