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American Chemical Society, Langmuir, 24(24), p. 13957-13961, 2008

DOI: 10.1021/la802075t

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A Two-Step Method to Covalently Bind Biomolecules to Group-IV Semiconductors: Si(111)/1,2-Epoxy-9-decene/Esterase

Journal article published in 2008 by Claire Jeanquartier, Gerburg Schider, Sabine Feichtenhofer, Helmut Schwab, Robert Schennach, Johanna Stettner, Adolf Winkler, Heidrun Gruber-Woelfler, Georg Schitter, Rafael J. P. Eder, Johannes G. Khinast
This paper is available in a repository.
This paper is available in a repository.

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Abstract

A versatile two-step method has been developed that allows linking of biomolecules covalently to hydrogen-terminated group-IV semiconductors by means of epoxy-alkenes. First, the terminal C==C double bond of the alkene forms a covalent bond with the silicon, germanium, or diamond surface by UV-mediated hydrosilylation. The terminal oxirane moiety then reacts with the biomolecule. As a model system, we investigated the attachment of an esterase B to a Si(111) surface by means of the linker molecule 1,2-epoxy-9-decene. Samples were characterized by X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared (FTIR) spectroscopy. The immobilized enzyme retained its activity and exhibited good long-term stability.