Fusarium oxysporum f.sp. radicis lycopersici (FORL) causes an important disease in tomato characterized by cell wall degradation. The characterization of cell wall-degrading polygalacturonase enzymes and their regulation is crucial to gain knowledge on the role of these enzymes in pathogenesis. The objectives of this work were to perform an in silico and in vitro analyses of the upstream regions of two exopolygalacturonase (EXOPG) coding genes of FORL and to develop an easy and reliable system to perform the functional analysis of those promoters. The analysis of the upstream regions of pgx1 and pgx2 genes revealed differences in the distribution of regulatory motifs. These upstream regions were fused with the beta-galactosidase reporter gene in a Saccharomyces cerevisiae vector. The induction of the reporter gene was analyzed in response to different carbon sources and the results compared to the induction pattern of pgx1 and pgx2 genes in in vitro cultures of FORL.