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Wiley, Yeast, 6(15), p. 459-472

DOI: 10.1002/(sici)1097-0061(199904)15:6<459::aid-yea387>3.0.co;2-l

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Two-Dimensional analysis of proteins secreted bySaccharomyces cerevisiae regenerating protoplasts: a novel approach to study the cell wall

This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Protoplasts of Saccharomyces cerevisiae incubated in regenerating conditions secrete cell wall components in order to allow the biosynthesis of this structure. During the first hours of incubation, many of these are not retained in the forming cell wall but remain in the medium. We have developed a method for collecting the secreted proteins and have analysed these by two-dimensional electrophoresis to obtain a reference map of putative cell wall proteins. Several proteins were identified by microsequencing or immunoblotting; namely, cell wall hydrolytic enzymes, heat shock proteins, glycolytic enzymes and others. Some β-1,3- and β-1,6-glucosylation was detected in the proteins secreted by regenerating protoplasts. Copyright © 1999 John Wiley & Sons, Ltd.