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Elsevier, Diagnostic Microbiology and Infectious Disease, 1(59), p. 101-104, 2007

DOI: 10.1016/j.diagmicrobio.2007.04.003

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Synthetic genes: a tool for identifying human papillomavirus genotypes by hybridization and polymerase chain reaction-based assays

Journal article published in 2007 by Karen Orjuela, Catalina Amador ORCID, Manuel A. Patarroyo ORCID
This paper is available in a repository.
This paper is available in a repository.

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Abstract

Obtaining positive polymerase chain reaction (PCR) controls for human papillomavirus (HPV) diagnostic tests has been difficult because of prevalence variation in different geographic regions of each high-risk viral type. Overlapping oligonucleotides were designed for HPV-18, HPV-31, HPV-45, and HPV-58 type-specific (TS) sequences. Synthetic HPV viral genes were constructed by 2-step assembly PCR for accurately diagnosing TS HPV infection.