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Nature Research, Nature Methods, 3(5), p. 239-241, 2008

DOI: 10.1038/nmeth.1181

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A systematic library for comprehensive overexpression screens in Saccharomyces cerevisiae

This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Modern genetic analysis requires the development of new resources to systematically explore gene function in vivo. Overexpression screens are a powerful method to investigate genetic pathways, but the goal of routine and comprehensive overexpression screens has been hampered by the lack of systematic libraries. Here we describe the construction of a systematic collection of the Saccharomyces cerevisiae genome in a high-copy vector and its validation in two overexpression screens.