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An Improved Procedure for the Enrichment of Plasma F (2)-Isoprostanes Prior to Final Determination by GC-MS/NICI

Journal article published in 2010 by J. Nourooz Zadeh
This paper was not found in any repository; the policy of its publisher is unknown or unclear.
This paper was not found in any repository; the policy of its publisher is unknown or unclear.

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Abstract

One of the most popular approaches to quantify oxidative injury is to measure lipid peroxidation products and, in particular, F2-isoprostanes (F2-IPs). F2-IPs is a group of prostaglandin F 2 -like compounds derived from the non-enzymatic oxidation of arachidonic acid. Of these, the 15-F 2t -isoprostane (8-iso-PGF 2) has received considerable attention, because it possesses adverse biological activities. Previous Gas Chromatographic-Mass Spectrometric (GC-MS) methods for measuring plasma F 2 -IPs from this laboratory involved two chromatography steps on C 18 and NH 2 -cartridges. Problems may, however, arise with chromatography on C18 cartridges, as it can be time-consuming and losses may occur depending upon the pH and eeciency of the sample loading. Therefore, it was decided that the C18 chromatography step be replaced with a single lipid partitioning step and the NH 2 -chromatography be simpliied. In 70 plasma samples from healthy individuals, total (sum of free and esteriied) 15-F 2t -isoprostane concentrations ranged from 0.5 to 3.13 nM. This assay meets all predeened method performances in terms of speciicity and sensitivity. The improved method is suitable for the analysis of samples from larger clinical trials investigating the role of oxidant injury under conditions associated with oxidative stress.