Copper (Cu) availability to plants depends on a large number of factors and their interactive relationships. Especially, localized excess soil Cu is often a great risk for plant growth on the agricultural lands. Development of resistant varieties to Cu toxicity is a high priority on these regions, where the soils have high levels of Cu. Thus, this study aimed to assess the resistance of wheat genotypes to Cu toxicity using the agronomic and physiologic parameters. For this aim, a pot experiment, based on a completely randomized design with three replications, was conducted using the soil of calcareous usthochrepts. In the study, twenty different wheat genotypes of T. aestivum and T. durum were used. Copper fertilizer at the levels of 0 (-Cu) and 20 mg Cu kg -1