The maskless and resistless focused ion beam (FIB) fabrication approach to make imprint stamp is straightforward and rapid compared to the traditional electron beam method. FIB etched stamp consisting of grooves was employed to nanoimprint polymer mr-I 9020. Taguchi orthogonal experiment with four parameter elements, one at three levels was used to optimize the experiment parameters by the analysis of means and variances. The most significant factor influencing the height of replicated lines is imprint temperature and the optimal combination of the process parameters are the imprint temperature at 160°C, imprint force at 1200N, loading force velocity at 0.2mm/min, and imprint time at 300s.