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American Chemical Society, Journal of Proteome Research, 2(7), p. 780-785, 2008

DOI: 10.1021/pr0705340

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Heat-Shock Response inArabidopsis thalianaExplored by Multiplexed Quantitative Proteomics Using Differential Metabolic Labeling

Journal article published in 2008 by Magnus Palmblad ORCID, Davinia J. Mills, Laurence V. Bindschedler
This paper is available in a repository.
This paper is available in a repository.

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Abstract

We have developed a general method for multiplexed quantitative proteomics using differential metabolic stable isotope labeling and mass spectrometry. The method was successfully used to study the dynamics of heat-shock response in Arabidopsis thaliana. A number of known heat-shock proteins were confirmed, and some proteins not previously associated with heat shock were discovered. The method is applicable in stable isotope labeling and allows for high degrees of multiplexing.