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In this study, we develop fluorescent protein probes specific for parallel G-quadruplexes by incorporating cyan fluorescent protein (CFP) to a G-quadruplex-binding motif of the RNA helicase associated with AU-rich element (RHAU). Fluorescent protein probes consisting of RHAU peptides of variable lengths were constructed and their binding to parallel G-quadruplexes was characterized. The selective recognition and discrimination of G-quadruplex topologies by the fluorescent protein probes could be easily detected by naked eyes or normal Gel Doc imaging.