In this paper, we present a one-step aqueous self-polymerization strategy to prepare surface molecularly imprinted poly(dopamine) (PDA) film. The method is simple and convenient compared with the “template-guided” surface molecularly imprinted synthesis method and the layer-by-layer self-assembly and the surface-grafted molecularly imprinted synthesis methods. The molecularly imprinted PDA films could selectively bind the template molecules (4-Hydroxybenzoic acid as a model), and bisphenol A (BPA) imprinted PDA films were also investigated. Meanwhile, the 2-dimension imprinted film material was used very conveniently.