The aim of this study was to evaluate the anterior temporalis and suprahyoid electromyographic (EMG) activity during jaw clenching and tooth grinding at different jaw posture tasks. The study included 30 healthy subjects with natural dentition and bilateral molar support, incisive protrusive guidance and bilateral laterotrusive canine guidance. Bipolar surface electrodes were located on the right anterior temporalis and suprahyoid muscles. Three EMG recordings in the standing position were performed in the following tasks: C. clenching in the intercuspal position (IP); P1. eccentric grinding from IP to protrusive edge-to-edge contact position; P2. clenching in protrusive edge-to-edge contact position; P3. concentric grinding from protrusive edge-to-edge contact position to IP; L1. eccentric grinding from IP to laterotrusive edge-to-edge contact position; L2. clenching in laterotrusive edge-to-edge contact position; L3. concentric grinding from laterotrusive edge-to-edge contact position to IP. EMG activity during protrusive and laterotrusive tasks was lower than intercuspal position in the anterior temporalis, whereas an opposite EMG pattern was observed in the suprahyoid muscles activity, excepting recorded activity in L2 (mixed model with unstructured covariance matrix). Anterior temporalis activity was higher during P3 than P1 and P2 tasks and during L3 than L1 and L2 tasks, whereas in the suprahyoid muscles, activity was higher during P1 than P2 tasks and during L1 than L2 and L3 tasks. These results could support the idea of a differential modulation of the motor neuron pools of anterior temporalis and suprahyoid muscles of peripheral and/or central origin.