Aloe vera L. (Aloeaceae) has been extensively studied for anti-inflammatory, antimicrobial, and cellular regeneration properties. This work evaluated in vivo the effects of pow-der of freeze-dried Aloe vera on rat pulp tissue. Pulp tissue was mechanically exposed to Aloe vera, and it was evalu-ated at 1 to 30 days after the procedure by histopathologic examination. A predominant acute-moderate inflammatory infiltrate was observed in the Aloe vera–treated group 1 to 7 days after treatment. At 14 to 30 days, pulp tissue took a normal pattern when comparing Aloe vera treatment with the Ca(OH) 2 treatment; it was found that both treatment groups stimulated reparative dentin and the formation of complete bridge. Strong superficial necrosis was detected exclusively for Ca(OH) 2 . It seems evident that application of Aloe vera in direct contact with the exposed pulp has acceptable biocompatibility and can lead to tertiary bridge formation.