The Portuguese Vinhos Verdes DOC Region retains a considerable biodiversity in grapevine varieties. Unfortunately , regional grapevine patrimony is in danger of disappearing. The present study was carried out in order to genotype, using 12 microsatellite loci, a group of 39 accessions of Vinhos Verdes DOC region, including underuse and minor varieties included in the wine list of varieties and also several unknown accessions with regional designations. The accessions analyzed were identified and grouped into 34 different genotypes , nine of them referred as new genotypes. Some new synonyms were detected, namely between Spanish and Portuguese cultivars. Misidentifications and wrong designations were also detected.