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Elsevier, Journal of Neuroimmunology, 1-2(196), p. 181-187, 2008

DOI: 10.1016/j.jneuroim.2008.03.009

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Neuromyelitis optica and anti-aquaporin-4 antibodies measured by an enzyme-linked immunosorbent assay

Journal article published in 2008 by Sei Hayakawa, Masahiro Mori, Akiko Okuta, Akiko Kamegawa, Yoshinori Fujiyoshi, Yasumasa Yoshiyama, Kaoru Mitsuoka, Kenichi Ishibashi, Sei Sasaki, Takamichi Hattori, Satoshi Kuwabara ORCID
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

NMO-IgG, a disease-specific autoantibody for neuromyelitis optica, recognizes aquaporin-4 (AQP4) and has been examined by indirect immunofluorescence assay. We developed an enzyme-linked immunosorbent assay (ELISA) to detect anti-AQP4 antibodies by establishing methods for expression in a baculovirus system and purification of recombinant AQP4 as antigen. Elevated anti-AQP4 antibody titers in serum were found in 15 (71%) of 21 patients with neuromyelitis optica, 4.3% of 46 patients with multiple sclerosis, none of 51 normal controls, and 2.6% of 115 patients with other neurological diseases. The ELISA system can be substituted for the conventional NMO-IgG assay.