Clonostachys rosea is a promising mycoparasite. In this study, we sequenced the draft genome of the highly effective strain 67-1 using the Illumina HiSeq 2500 sequencing platform. The genome is 55.4 Mb with a G+C content of 49.2% and provides a powerful resource for future studies on the molecular mechanisms underlying Clonostachys rosea’s antagonism on fungal pathogens. FOOTNOTES Address correspondence to Man-Hong Sun, sunmanhong2013{at}163.com, or Shi-Dong Li, lisd{at}ieda.org.cn. Citation Sun Z-B, Sun M-H, Li S-D. 2015. Draft genome sequence of mycoparasite Clonostachys rosea strain 67-1. Genome Announc 3(3):e00546-15. doi:10.1128/genomeA.00546-15. Received 22 April 2015. Accepted 28 April 2015. Published 28 May 2015. Copyright © 2015 Sun et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 3.0 Unported license.