Taylor and Francis Group, Connective Tissue Research, 4(56), p. 315-325, 2015
DOI: 10.3109/03008207.2015.1027340
Full text: Unavailable
Aim of the study The molecular aspects of inflammation were investigated in equine articular cartilage explants using quantitative proteomics. Material and Methods Articular cartilage explants were stimulated with interleukin (IL)-1β in vitro for 25 days, and proteins released into cell culture media were chemically labelled with isobaric mass tags and analysed by liquid chromatography-tandem mass spectrometry. Results A total of 127 proteins were identified and quantified in media from explants. IL-1β-stimulation resulted in an abundance of proteins related to inflammation, including matrix metalloproteinases, acute phase proteins, complement components and IL-6. Extracellular matrix (ECM) molecules were released at different time points and fragmentation of aggrecan and cartilage oligomeric matrix protein was observed at days 3 and 6, similar to early-stage OA in vivo. Degradation products of the collagenous network were observed at days 18 and 22, similar to late-stage OA. Conclusion This model displays a longitudinal quantification of released molecules from the ECM of articular cartilage. Identification of dynamic changes of extra cellular matrix molecules in the secretome of equine explants stimulated with IL-1β over time may be useful for identifying components released at different time points during the spontaneous OA process.