This experiment was carried out to determine the effects of using L-carnitine and humate alone or in combination in quail diets on laying performance, egg traits and blood parameters. A total of 280 Japanese quails aged 10 weeks, divided into one control group and three treatment groups, were used. The diets of the first, second and third treatment groups were supplemented with 100 mg L-carnitine/kg, 1.5 g humate (Farmagülatör^® Dry Plus)/kg and 100 mg L-carnitine + 1.5 g humate/kg, respectively. The experimental period lasted 16 weeks. The addition of L-carnitine and sodium humate alone or in combination did not significantly affect body weight, feed consumption, egg production, feed conversion ratio, mortality, egg-shell thickness, egg yolk index and the percentages of egg-shell, albumen and yolk. Egg weight increased (P < 0.001) with L-carnitine supplementation. The values of egg albumen height (P < 0.05), egg albumen index (P < 0.01) and egg Haugh unit (P < 0.05) were increased with humate supplementation. Egg cholesterol content and blood serum parameters were not affected by the supplementation of L-carnitine with or without humate. The results in this study demonstrated that L-carnitine supplementation increased egg weight while humate addition increased egg albumen index and egg Haugh unit of laying quails. However, the combined administration of L-carnitine and humate did not have any significant effects on the parameters measured.