The Triple Data Encryption Algorithm (Triple DES) is an international standard of block cipher, which composed of two encryption processes and one decryption process of DES to increase security level. In this paper, we proposed a Differential Fault Analysis (DFA) attack to retrieve secret keys using reduction of last round execution for each DES process in the Triple DES by fault injections. From the simulation result for the proposed attack method, we could extract three 56-bit secret keys using exhaustive search attack for candidate keys which are refined from about 9 faulty-correct cipher text pairs. Using laser fault injection experiment, we also verified that the proposed DFA attack could be applied to a pure microprocessor ATmega 128 chip in which the Triple DES algorithm was implemented.