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Elsevier, Stem Cell Research, 3(11), p. 1378-1392, 2013

DOI: 10.1016/j.scr.2013.09.005

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Chick stem cells: Current progress and future prospects

Journal article published in 2013 by Sittipon Intarapat, Claudio D. Stern ORCID
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Chick embryonic stem cells (cESCs) can be derived from cells obtained from stage X embryos (blastoderm stage); these have the ability to contribute to all somatic lineages in chimaeras, but not to the germ line. However, lines of stem cells that are able to contribute to the germ line can be established from chick primordial germ cells (cPGCs) and embryonic germ cells (cEGCs). This review provides information on avian stem cells, emphasizing different sources of cells and current methods for derivation and culture of pluripotent cells from chick embryos. We also review technologies for isolation and derivation of chicken germ cells and the production of transgenic birds.