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BioMed Central, BMC Research Notes, 1(1), p. 129

DOI: 10.1186/1756-0500-1-129

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An Italian functional genomic resource for Medicago truncatula

Journal article published in 2008 by Andrea Porceddu, Francesco Panara ORCID, Lorna Molinari, Paola Taviani, Luisa Lanfaloni, Carla Scotti, Maria Carelli, Laura Scaramelli, Viviane Cosson, P. Cosson, Pascal Ratet, Henri de Larembergue, Henri de Larembergue, Henri De Larambergue, Gerard Duc and other authors.
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Background : Medicago truncatula is a model species for legumes. Its functional genomics have been considerably boosted in recent years due to initiatives based both in Europe and US. Collections of mutants are becoming increasingly available and this will help unravel the genetic control of important traits for many species of legumes. Findings : Our report is on the production of three complementary mutant collections of the model species Medicago truncatula produced in Italy in the frame of a national genomic initiative. Well established strategies were used: Tnt1 mutagenesis, TILLING and activation tagging. Both forward and reverse genetics screenings proved the efficiency of the mutagenesis approaches adopted, enabling the isolation of interesting mutants which are in course of characterization. We anticipate that the reported collections will be complementary to the recently established functional genomics tools developed for Medicago truncatula both in Europe and in the United States.