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Oxford University Press (OUP), Chemical Senses, 5(35), p. 363-373

DOI: 10.1093/chemse/bjq024



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Molecular Characterization of a Phospholipase C Potentially Involved in Moth Olfactory Transduction

This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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To clarify the role of phospholipase C (PLC) in insect olfactory transduction, we have undertaken its molecular identification in the moth Spodoptera littoralis. From the analysis of a male antennal expressed sequence tag library, we succeeded in cloning a full-length cDNA encoding a PLC that belongs to the cluster of PLC-beta subtypes. In adult males, the PLC-beta transcript was located predominantly in brain and antennae where its presence was detected in the olfactory sensilla trichodea. Moreover, PLC-beta was expressed in antennae at the beginning of the pupal stage, then reached a maximum at the end of this stage and was maintained at this level during the adult period. Taken together, these results provided molecular evidence for the putative participation of a PLC-beta in signaling pathways responsible for the establishment and the functioning of insect olfactory system.