The purpose of this paper is to prove that near infrared radiation (NIR) modifies hydrogen bonds localized in the interface of reversed micelles. The degree of modification of the hydrogen bonds was monitored by TEMPO-palmitate spin probe introduced into the structure of reverse micelles formed by cetyltrimethylammonium bromide (CTAB) (TCAB/phosphate buffer/isooctane/hexanol and TCAB/NaCl/isooctane/hexanol (W = 15)). Electron paramagnetic resonance (EPR) spectra were performed on the argonated samples. The isotropic tumbling correlation time (τc) and the hyperfine coupling constant A+ = h0 – h+1 (h+1, and h0 correspond to the low-, and centre-field lines, respectively) were determined from the EPR spectra as a quantitative measure for monitoring the action of NIR radiation. A+ values depend on the composition of the water pool (1.640 mT for phosphate buffer and 1.630 mT for NaCl). NIR irradiation led to decrease in A+. This parameter reached the same value for both solutions (1.625 ± 0.003 and 1.626 ± 0.003 mT) after exposition to NIR. The tumbling correlation time after exposure to NIR decreased for TCAB/phosphate buffer/ isooctane/hexanol reversed micelles from 2.10 × 10–10 s to 1.44 × 10–10 s but did not change for TCAB/NaCl/isooctane/ hexanol). The results obtained confirm the possibility of modification of the hydrogen bonds by NIR radiation.