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Centers for Disease Control and Prevention, Emerging Infectious Diseases, 6(13), p. 899-901

DOI: 10.3201/eid1306.061572

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Loop-Mediated Isothermal Amplification for Influenza A (H5N1) Virus

Journal article published in 2007 by Shanthi Jayawardena, Chung Y. Cheung, Ian Barr, Kwok H. Chan, Honglin Chen, Yi Guan, J. S. Malik Peiris, Leo L. M. Poon ORCID
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

We describe a 1-step reverse-transcription loop-mediated isothermal amplification assay for detection of highly pathogenic avian influenza A (H5N1) viruses. The assay was tested by using a panel of highly pathogenic H5N1 subtypes isolated over the past 10 years and clinical specimens. The assay produced negative results for all non-H5N1 subtypes.