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Nature Research, Nature Biotechnology, 9(26), p. 1015-1017, 2008

DOI: 10.1038/nbt.1491

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T-DNA–mediated transfer of Agrobacterium tumefaciens chromosomal DNA into plants

Journal article published in 2008 by Bekir Ulker, Bekir Ülker, Yong Li ORCID, Mario G. Rosso, Elke Logemann, Imre E. Somssich, Bernd Weisshaar ORCID
This paper is available in a repository.
This paper is available in a repository.

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Abstract

Besides the well-documented integration of DNA flanked by the transfer DNA borders, occasional insertion of fragments from the tumor-inducing plasmid into plant genomes has also been reported during Agrobacterium tumefaciens-mediated transformation. We demonstrate that large (up to approximately 18 kb) gene-bearing fragments of Agrobacterium chromosomal DNA (AchrDNA) can be integrated into Arabidopsis thaliana genomic DNA during transformation. One in every 250 transgenic plants may carry AchrDNA fragments. This has implications for horizontal gene transfer and indicates a need for greater scrutiny of transgenic plants for undesired bacterial DNA.