Modern structures are designed to withstand in-service loads over a broad frequency spectrum. Nonetheless, mechanical properties in numerical codes are assumed to be frequency-independent to simplify calculations or due to a lack of experimental data, and this approach could lead to overdesign or failures. This study aims to quantify the frequency effects in the fatigue applications of a bi-material adhesive joint through analytical, numerical, and experimental procedures. Analytical and finite element models allowed the specimen design, whereas the frequency effects were investigated through a conventional servo-hydraulic apparatus at 5, 25, and 50 Hz and with an ultrasonic fatigue testing machine at 20 kHz. Experimentally, the fatigue life increases with the applied test frequency. Run-out stress data at 109 cycles follow the same trend: at 25 Hz and 50 Hz, the run-out data were found at 10 MPa, increasing to 15 MPa at 20 kHz. The P–S–N curves showed that frequency effects have a minor impact on the experimental variability and that standard deviation values lie in the range of 0.3038–0.7691 between 5 Hz and 20 kHz. Finally, the trend of fatigue strengths at 2·106 cycles with the applied loading frequency for selected probability levels was estimated.