Abstract Modric, T, Esco, M, Perkovic, S, Basic, Z, Versic, S, Morgans, R, and Sekulic, D. Artificial turf increases the physical demand of soccer by heightening match running performance compared with natural grass. J Strength Cond Res 37(11): 2222–2228, 2023—This study aimed to determine differences in match running performance (MRP) of elite soccer players (n = 31) during matches played on artificial turf (AT) and natural grass (NG). The players' MRP (AT; 131 observations, NG; 135 observations) were collected during official matches (n = 32) using global positioning system and classified according to their playing positions as central defenders, central midfielders, fullbacks, forwards, and wingers. The MRP variables included total distance covered (TD), low-intensity running (LIR), moderate-intensity running (MIR), high-intensity running (HIR), total (Tacc) and high-intensity accelerations (Hacc), and total (Tdec) and high-intensity decelerations (Hdec). The influence of match outcome, location, and opponent quality was controlled for as contextual factors. Playing matches on AT resulted in (a) increased TD (Cohen's d (d) = 0.55, 0.61, and 0.28, respectively), MIR (d = 0.91, 0.79, and 0.49, respectively), and HIR (d = 0.67, 0.44, and 0.38, respectively) for central defenders, central midfielders, fullbacks; (b) increased Tacc (d = 0.38, 0.37, and 0.4, respectively) and Tdec (d = 0.31, 0.34, and 0.31, respectively) for central defenders, central midfielders, and wingers; and (c) decreased Hdec (d = −1.5) for forwards. These results show that playing on AT is more physically demanding for defensive and midfield players than playing on NG. Soccer coaches should consider implementing preparatory strategies that mitigate the increased demands of playing on AT for these players, especially before a competitive period when transitions between pitch surfaces routinely occur.