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AOAC International, Journal of AOAC INTERNATIONAL, 2021

DOI: 10.1093/jaoacint/qsab135

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Emergency Response Validation of the Soleris® Direct Yeast and Mold Method for Detection of Yeast and Mold in Dried Cannabis Flower: AOAC Performance Tested MethodSM 051301

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Distributing this paper is prohibited by the publisher

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Abstract

Abstract Background Soleris® Direct Yeast and Mold is a growth-based, automated method for detection of yeast and mold in select foods and other sample types including nutraceuticals and cosmetics. The Soleris method is used in a “dilute-to-specification” or threshold manner in which a result is scored as positive or negative around a predetermined cutoff (in CFU/g) established by the dilution and volume of sample homogenate tested. Objective The objective of this study was to validate the method for testing of dried cannabis flower. The validation was conducted under the Emergency Response Validation program of the AOAC Research Institute. Methods The study included inclusivity and exclusivity testing, in particular testing of yeast and mold species associated with cannabis, and a matrix study in which Soleris method presumptive results were compared to Soleris confirmed results using Dichloran Rose Bengal Chloramphenicol agar for confirmation. Samples at four different levels of natural yeast and mold contamination were tested at two test thresholds. Results In inclusivity testing, all 63 yeast and mold strains tested produced positive results within the specified test duration of 72 h. In exclusivity testing, 36 of 37 strains tested produced no detection within 72 h. In matrix testing, there were no significant differences between Soleris presumptive and confirmed results for any contamination level or test threshold as determined by probability of detection analysis. Conclusions and Highlights Results indicate that the Soleris method is an effective procedure for detection of yeast and mold in dried cannabis flower. Results are available within 72 h compared with the 5–7 days required for microbiological culture methods.