Due to superficial morphological similarities, there is often confusion in the identification of some species of the genus Septaria. A combined analysis of the genital anatomy, morphometric and DNA, based on a portion of the COI gene, applied to three nominal species of this genus, confirmed the validity of Septaria tahitiana Eichhorst, 2016, and suggested that Septaria borbonica (Bory de Saint Vincent, 1804) is a subspecies of Septaria porcellana (Linnaeus, 1758), both taxa with disjunct distribution areas: Septaria borbonica in the western Indian Ocean and Septaria porcellana the western Pacific and eastern Indian Oceans. The possible presence of Septaria tesselata (Lamarck, 1816) in Mayotte (Comoros Archipelago) needs to be confirmed.