During a survey on diversity of freshwater fungi along a north-south latitudinal gradient in Asia, three fresh specimens of Minimelanolocus were collected from submerged wood in streams in northwestern Yunnan Province, China. Based on their distinct morphological features and phylogenetic analyses of combined ITS, LSU and SSU sequence data, Minimelanolocus nujiangensis and M. clavatus are described as new species and M. submersus was recollected from Yunnan, China. Illustrations and descriptions with notes of the three species are provided. This study increases the known diversity of Minimelanolocus and enriches freshwater fungal resources.