The caddisfly species Rhyacophila pubescens Pictet, 1834 is reported from R. North Macedonia for the first time. Our investigations showed the presence of this species at only one locality at Osogovo Mountain, above the Sasa mine. R. pubescens is commonly distributed in Central Europe but has been rarely found in the southeastern parts of the continent. This record marks the southernmost occurrence of this species in the Balkan Peninsula and increases the number of Rhyacophila species of R. North Macedonia to seventeen. The results obtained in our study a) provide information on the distribution of R. pubescens in the Balkan Peninsula; b) comment on the ecological preferences of the species and c) serve as a valuable source of information for further biodiversity and population studies.