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Cold Spring Harbor Laboratory Press, Genome Research, 7(11), p. 1296-1303, 2001

DOI: 10.1101/gr.177601

Cold Spring Harbor Laboratory Press, Genome Research, 7(11), p. 1296-1303

DOI: 10.1101/gr.gr-1776r

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Massive Sequence Comparisons as a Help in Annotating Genomic Sequences

This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Data provided by SHERPA/RoMEO

Abstract

An all-by-all comparison of all the publicly available protein sequences from plants has been performed, followed by a clusterization process. Within each of the 1064 resulting clusters—containing sequences that are orthologous as well as paralogous—the sequences have been submitted to a pyramidal classification and their domains delineated by an automated procedure à la PRODOM. This process provides a means for easily checking for any apparent inconsistency in a cluster, for example, whether one sequence is shorter or longer than the others, one domain is missing, etc. In such cases, the alignment of the DNA sequence of the gene with that of a close homologous protein often reveals (in 10% of the clusters) probable sequencing errors (leading to frameshifts) or probable wrong intron/exon predictions. The composition of the clusters, their pyramidal classifications, and domain decomposition, as well as our comments when appropriate, are available from http://chlora.infobiogen.fr:1234/PHYTOPROT.