Published in
Royal Society of Chemistry, Nanoscale, 35(11), p. 16270-16276, 2019
DOI: 10.1039/c9nr04460d
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Enhancing the stability of DNA origami nanostructures: staple strand redesignversusenzymatic ligation
Journal article published in 2019 by
Saminathan Ramakrishnan,
Leonard Schärfen 
,
Kristin Hunold,
Sebastian Fricke,
Guido Grundmeier,
Michael Schlierf ,
Adrian Keller ,
Georg Krainer
,
Kristin Hunold,
Sebastian Fricke,
Guido Grundmeier,
Michael Schlierf ,
Adrian Keller ,
Georg Krainer
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Abstract
Merging of bridging staples with adjacent oligonucleotide sequences leads to a moderate increase of DNA origami stability, while enzymatic ligation after assembly yields a reinforced nanostructure with superior stability at up to 37 °C and in the presence of 6 M urea.