Rondonops biscutatus is a gymnophthalmid litter-lizard which occurs in the Amazon rainforests and the transitional areas between Amazonia and Cerrado. This species’ distribution is strongly biased by a lack of data, possibly because it was recently described, small-bodied, and living in cryptic habits. Here, we present occurrence data of six specimens from four locations in transitional areas between forests in Amazonia and Cerrado (central Brazil), in the upper Paraguay River, at the northern end of the Pantanal, midwestern Brazil. We expand the distribution of this species 507 km east from its original range.​