Published in

Rockefeller University Press, Journal of Cell Biology, 3(173), p. 349-360, 2006

DOI: 10.1083/jcb.200510080

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A functional network involved in the recycling of nucleocytoplasmic pre-60S factors

Journal article published in 2006 by Alice Lebreton, Cosmin Saveanu, Laurence Decourty, Jean-Christophe Rain, Alain Jacquier ORCID, Micheline Fromont-Racine ORCID
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Eukaryotic pre-ribosomes go through cytoplasmic maturation steps before entering translation. The nucleocytoplasmic proteins participating in these late stages of maturation are reimported to the nucleus. In this study, we describe a functional network focused on Rei1/Ybr267w, a strictly cytoplasmic pre-60S factor indirectly involved in nuclear 27S pre-ribosomal RNA processing. In the absence of Rei1, the nuclear import of at least three other pre-60S factors is impaired. The accumulation in the cytoplasm of a small complex formed by the association of Arx1 with a novel factor, Alb1/Yjl122w, inhibits the release of the putative antiassociation factor Tif6 from the premature large ribosomal subunits and its recycling to the nucleus. We propose a model in which Rei1 is a key factor for the coordinated dissociation and recycling of the last pre-60S factors before newly synthesized large ribosomal subunits enter translation.