We describe a new species of diurnal gecko, Gonatodes machelae sp. nov., from Cerro El Copey, Margarita Island, Venezuela. The new species is distinguished from all congeners by a combination of the following characters: small size, subcaudal pattern C (1’1’’), typically three lateral scale rows on the digits, and aspects of coloration. The new species exhibits polychromatism similar to what has been described in some other species of Gonatodes. It is found in humid forests and shrublands from about 450 m to 900 m above sea level.