Published in

American Society for Microbiology, Journal of Bacteriology, 19(193), p. 5242-5251, 2011

DOI: 10.1128/jb.05429-11

Links

Tools

Export citation

Search in Google Scholar

Role for Escherichia coli YidD in Membrane Protein Insertion

Journal article published in 2011 by Zhong Yu, Mariëlle Laven, Mirjam Klepsch, Jan-Willem de Gier, Wilbert Bitter ORCID, Peter van Ulsen, Joen Luirink ORCID
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

Full text: Download

Green circle
Preprint: archiving allowed
Upload
Green circle
Postprint: archiving allowed
Upload
Orange circle
Published version: archiving restricted
Upload
Policy details
Data provided by SHERPA/RoMEO

Abstract

ABSTRACT YidC has an essential but poorly defined function in membrane protein insertion and folding in bacteria. The yidC gene is located in a gene cluster that is highly conserved in Gram-negative bacteria, the gene order being rpmH , rnpA , yidD , yidC , and trmE . Here, we show that Escherichia coli yidD , which overlaps with rnpA and is only 2 bp upstream of yidC , is expressed and localizes to the inner membrane, probably through an amphipathic helix. Inactivation of yidD had no discernible effect on cell growth and viability. However, compared to control cells, Δ yidD cells were affected in the insertion and processing of three YidC-dependent inner membrane proteins. Furthermore, in vitro cross-linking showed that YidD is in proximity of a nascent inner membrane protein during its localization in the Sec-YidC translocon, suggesting that YidD might be involved in the insertion process.