Published in

American Association for the Advancement of Science, Science, 6407(361), p. 1122-1126, 2018

DOI: 10.1126/science.aat7992

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Semisynthetic sensor proteins enable metabolic assays at the point of care

This paper was not found in any repository, but could be made available legally by the author.
This paper was not found in any repository, but could be made available legally by the author.

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Abstract

A protein designed to sense metabolites Many diseases cause characteristic changes in blood metabolites. Yu et al. describe a paper-based assay in which a chosen metabolite can be oxidized to generate reduced nicotinamide adenine dinucleotide phosphate (NADPH). Color changes in a designed NADPH sensor protein are then quantified by a digital camera. The sensor system successfully generated point-of-care measurements of phenylalanine, glucose, and glutamate. Concentrations of phenylalanine in the blood of phenylketonuria patients were analyzed within minutes with only half a microliter of blood. Science , this issue p. 1122