Introduction and Aim. Injecting drug users (IDUs) are amongst the most vulnerable people to acquire HIV/AIDS. This study aims to collect information on IDUs and their health seeking behavior in Bangladesh. Design and Methods. A cross-sectional study was conducted among 120 IDUs attending a drug rehabilitation center in Dhaka, Bangladesh. Data were collected on socio-demographics, drug use, health seeking behavior, knowledge of injecting drugs and sexual behavior. Results. The mean±SD and median (IQR) age of the participants were 32.5±21.3 and 33 (27-38) years, respectively with only 9.2% females. Injection buprenorphine was the drug of choice for 40% participants, while more than half (58%) of the participants first started drug use with smoking cannabis. 73.3% participants shared needles sometimes and 57.5% were willing to use the Needle Exchange Programs. 60% of the participants had no knowledge about the diseases spread by injection. Only 63.3% participants mentioned to have sex with regular partners. Condom use during last intercourse with regular partners was 11.7% and with any partners15.8%. Conclusion. IDUs in Bangladesh are a high risk group for HIV/AIDS due to lack of knowledge and risky behaviors. Education and interventions specifically aimed at IDUs are needed, because education targeted at the general population may not reach IDUs or influence their behavior.