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Oxford University Press, Nucleic Acids Research, 5(36), p. 1532-1541, 2008

DOI: 10.1093/nar/gkm1017

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Stretching and unzipping nucleic acid hairpins using a synthetic nanopore

Journal article published in 2008 by Q. Zhao, J. Comer, V. Dimitrov, S. Yemenicioglu, A. Aksimentiev ORCID, G. Timp
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

We have explored the electromechanical properties of DNA by using an electric field to force single hairpin molecules to translocate through a synthetic pore in a silicon nitride membrane. We observe a threshold voltage for translocation of the hairpin through the pore that depends sensitively on the diameter and the secondary structure of the DNA. The threshold for a diameter 1.5 1.5 V, which corresponds to the force required to stretch the stem of the hairpin, according to molecular dynamics simulations. On the other hand, for 1.0