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ABSTRACT The genome of human papillomavirus type 23 (HPV-23; family Papillomaviridae , genus Betapapillomavirus , species Betapapillomavirus 2 , type 23) was assembled by deep sequencing from nasopharyngeal swabs. The assembled genome is 2.7% divergent over its full length from the single complete genome of HPV-23 in GenBank (accession no. U31781). We named the strain HPV-23/Lancaster/2015.