@article{Coelho2015, abstract = {Mice deficient in adipose triglyceride lipase (ATGL−/−) present elevated ectopic lipid levels but are paradoxically glucose-tolerant. Measurement of endogenous glucose production (EGP) and Cori cycle activity provide insights into the maintenance of glycemic control in these animals. These parameters were determined in 7 wild-type (ATGL+/−) and 6 ATGL−/−mice by a primed-infusion of [U-13C6]glucose followed by LC-MS/MS targeted mass-isotopomer analysis of blood glucose. EGP was quantified by isotope dilution of [U-13C6]glucose while Cori cycling was estimated by analysis of glucose triose13C-isotopomers. Fasting plasma free fatty-acids were significantly lower in ATGL−/−versus control mice (0.43 ± 0.05 mMversus0.73 ± 0.11 mM,P<0.05). Six-hour fasting EGP rates were identical for both ATGL−/−and control mice (79 ± 11versus71 ± 7 μmol/kg/min, resp.). Peripheral glucose metabolism was dominated by Cori cycling (80 ± 2% and 82 ± 7% of glucose disposal for ATGL−/−and control mice, resp.) indicating that peripheral glucose oxidation was not significantly upregulated in ATGL−/−mice under these conditions. The glucose13C-isotopomer distributions in both ATGL−/−and control mice were consistent with extensive hepatic pyruvate recycling. This suggests that gluconeogenic outflow from the Krebs cycle was also well compensated in ATGL−/−mice.}, author = {Coelho, Margarida and Nunes, Patricia and Mendes, Vera M. and Manadas, Bruno and Heerschap, Arend and Jones, John G.}, doi = {10.1155/2015/542029}, journal = {Journal of Diabetes Research}, month = {jan}, pages = {1-8}, title = {Effect of Global ATGL Knockout on Murine Fasting Glucose Kinetics}, url = {https://doi.org/10.1155/2015/542029}, volume = {2015}, year = {2015} }