@article{Meulenbelt1993, abstract = {We have explored the usefulness of a cloned low-repetitive DNA element, termed RTVL-H, for the region-specific isolation of DNA cosmids from chromosome 21. Hybridization of the RTVL-H element as a probe to genomic Southern blots of DNAs from a chromosome 21-specific somatic cell hybrid panel demonstrated the presence of at least seven RTVL-H-related sequences in defined regions dispersed over the chromosome. Some of the RTVL-H sequences detected by using the chromosome 21-specific somatic cell hybrid panel were subsequently isolated from a chromosome 21-specific cosmid library and mapped using somatic cell hybrids. In general, close correspondence was found between mapping results obtained using the consensus RTVL-H probe on the chromosome 21-specific somatic cell hybrid panel and the more precise mapping obtained using cosmids. The results demonstrate that sequences such as the RTVL-H element can be used for isolating region-specific sequences.}, author = {Meulenbelt, Ingrid and Wapenaar, Martin C. and Patterson, David and Vijg, Jan and Uitterlinden, André G.}, doi = {10.1006/geno.1993.1099}, journal = {Genomics}, month = {mar}, pages = {492-499}, title = {Isolation and Mapping of Human Chromosome 21 Cosmids Using a Probe for RTVL-H Retrovirus-like Elements}, url = {https://www.researchgate.net/profile/Martin_Wapenaar/publication/14731356_Isolation_and_Mapping_of_Human_Chromosome_21_Cosmids_Using_a_Probe_for_RTVL-H_Retrovirus-like_Elements/links/55ad990c08aed9b7dcdae82a.pdf}, volume = {15}, year = {1993} }