@article{Dhillon2009, abstract = {Neuropeptide Y (NPY) regulates reproductive function at the level of the hypothalamus through control of GnRH secretion. However, the direct control of GnRH gene expression by NPY has not yet been studied. GT1-7 neurons were treated with 100 nM of NPY over a 36 h time course. GnRH mRNA levels were significantly increased by NPY up to 12 h. We determined that GT1-7 neurons expressed Y1, Y2, and Y4 NPY receptors, but not Y5. Functional analysis of NPY receptor activation indicated that the Y1/Y4/Y5 receptor agonist [Leu31, Pro34] significantly induced cAMP accumulation in the GT1-7 neurons. Western blot studies demonstrated changes in the phosphorylation status of AKT, ERK1/2, CREB and ATF-1 after NPY exposure. Pharmacological inhibitors of the MAPK and PKA signal transduction pathways attenuated the NPY-mediated increase in GnRH transcription. This NPY-mediated increase in GnRH mRNA was also inhibited with the Y1-receptor specific antagonist BIBP-3226. The mHypoE-38 neurons secrete detectable levels of NPY and can be used as an endogenous source of NPY. Conditioned medium from mHypoE-38 neurons induced an increase in GnRH mRNA, which was inhibited by the Y1 receptor antagonist BIBP-3226. Together, these studies strengthen the evidence for the importance of NPY in the regulation of reproductive function.}, author = {Dhillon, Sandeep S. and Gingerich, Sarah and Belsham, Denise D.}, doi = {10.1016/j.regpep.2009.04.005}, journal = {Regulatory Peptides}, month = {may}, pages = {96-103}, title = {Neuropeptide Y induces gonadotropin-releasing hormone gene expression directly and through conditioned medium from mHypoE-38 NPY neurons}, url = {https://www.researchgate.net/profile/Denise_Belsham/publication/24282653_Neuropeptide_Y_induces_gonadotropin-releasing_hormone_gene_expression_directly_and_through_conditioned_medium_from_mHypoE-38_NPY_neurons/links/55ad611508ae98e661a42b04.pdf}, volume = {156}, year = {2009} }