@article{Marinovich2015, abstract = {Tendons and ligaments insert into bone through a transitional tissue termed the enthesis which is susceptible to injury and difficult to repair. Entheses contain a region of calcified fibrocartilage (CFC), however mineral-associated proteins in this tissue remain poorly characterized. Bone sialoprotein (BSP) is a phosphoprotein associated with mineralizing tissues. In these studies BSP was identified in the CFC of entheses by immunohistochemistry. Analysis of the entheses of Bsp-/- mice indicate abnormalities in the CFC. Compared to controls, the CFC of the quadriceps tendon enthesis is 28% and 41 % longer in 15 week and 14 month old Bsp-/- mice, respectively. MicroCT and Raman spectroscopic analysis of the CFC in Bsp-/- mice demonstrate that mineral content is similar between genotypes. Mechanical studies show that the Bsp-/- patellar tendon is larger in cross-sectional area yet mechanically weaker. These data suggest BSP is involved in the regulation and growth of the CFC.}, author = {Marinovich, Ryan M. and Soenjaya, Yohannes and Wallace, Gregory Q. and Zuskov, Andre and Dunkman, Andrew and Foster, Brian L. and Ao, Min and Bartman, Kevin and Lam, Vida and Rizkalla, Amin and Beier, Frank and Somerman, Martha J. and Holdsworth, David W. and Soslowsky, Louis J. and Lagugné-Labarthet, François and Goldberg, Harvey A.}, doi = {10.1016/j.matbio.2016.01.016}, journal = {Matrix Biology}, month = {jul}, title = {The role of bone sialoprotein in the tendon-bone insertion}, url = {https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4875796}, year = {2015} }