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Elsevier, Journal of Chromatography B, (951-952), p. 119-128, 2014

DOI: 10.1016/j.jchromb.2014.01.011

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A versatile ultra-high performance LC-MS method for lipid profiling☆

Journal article published in 2014 by Oskar L. Knittelfelder ORCID, Knittelfelder Ol, Bernd P. Weberhofer, Weberhofer Bp, Thomas O. Eichmann ORCID, Eichmann To, Sepp D. Kohlwein, Kohlwein Sd, Rechberger Gn, Gerald N. Rechberger
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This paper is available in a repository.

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Abstract

A new UPLC-based untargeted lipidomic approach using a qTOF hybrid mass spectrometer is introduced. The applied binary gradient enables separations of lipid species including constitutional isomeric compounds and low abundant lipid classes such as phosphatidic acid (PA). Addition of phosphoric acid to the solvents improves peak shapes for acidic phospholipids. MSE scans allow simultaneous acquisition of full scan data and collision induced fragmentation to improve identification of lipid classes and to obtain structural information. The method was used to investigate the lipidome of yeast.